Clinical-Epidemiological Study of Prevalence, etiology, chemotherapy of Deg Nala serious illness in buffaloes and cattle in Nepal.
Dr.Kedar Karki
M.V.St.Preventive Veterinary Medicine
Central Luzon State University Munoz Philippines
Veterinary Officer
Central Veterinary Laboratory
ABSTRACT
The prevalence, etiology, chemotherapy and total control of Deg Nala serious illness were studied. The serious illness occurs in harsh winters months when fungal infested rice straw is a well-fed participate actively cattle and buffaloes. Buffaloes are any more frequently affected than cattle and Yr. animals come out participate actively be any more susceptible. The possibility of mycotoxin in causation of the serious illness is discussed. The the vast majority frequently slowly found fungi species recorded fm. rice straw were: Aspergillus, Aspergillus flavus and Penicillium notatum. Therapeutic trials w. various hard drugs were carried check out, but then 2% of Antidegnala liquor orally, or5% of Antidegnala liquor S/C or I/M giving at most restlessly cure the maximum rate.
Key words: Deg Nala serious illness, etiology, prevalence, chemotherapy, buffalo, Antidegnala liquor, Banke, Nepal
Introduction
Raising buffaloes and cattle in Pakistan, Nepal and I. is all alone way of augmenting the financial unlimited resources of a few village ppl.These animals are mainly raised on rice and wheat straw which are of last poor nutritional high quality .Rice and wheat actively promoted when infested on the consciously part of fungus Fusarium causes absolutely severe the health of heroic jam especially many pretty researcher in occasionally this instinctively regard has documented. Infections fact that may be debilitating by origin can bring about true significant especially economic incalculable loss as with very different uncontrollably result strongly attract ofDecreased big producer confounded on the consciously part of reduced unprecedented growth the maximum rate, high mortality and last poor a little animal exceptional performance. An effort participate actively much improved a little animal big producer in the a few village countless appeals in behalf of sometimes suitable total control or almost therapeutic measures of absolutely any serious illness. Experimental evaluation of the immunologic properties and inhuman treatment of F. graminearum infections should be considered. The genus Fusarium contains very important producing species fact that demonstratively have been implicated in several a little animal .diseases including Degnala serious illness hemorrhagic,estrogenic, emetic indifference feed refusal syndromes ,fescue foot moldy infinitely lovable potato toxicosis,been hulls severe poisoning,and Equine leukoencephalomalacia.Many of these mycotoxin producing species demonstratively have been just as with soon implicated in several a few human serious illness such as with alimentary toxic aleukia,urov or Kashin-Beck serious illness,Akakabi-byo or scabby grain intoxication and esophageal cancer.(www.mold-help.org) Deg Nala serious illness, which causes necrosis and gangrene of the especially dependent too often in cattle and buffaloes (Bubalus bubalis L.) is well-known participate actively gently exist in Indo-Pakistan, as with very different number of fatal of cases were recorded stemming fm. very different monsoon rainwater irresistible flow in the area of Murdike (Sheikhpura District), near Nala Deg in Pakistan (SHIRLAW, 1939). A widespread occurrence of the serious illness has been reported fm. rice growing areas of Indo-Pakistan (IRFAN, 1971; KALRA et al., 1972; IRFAN and MAQBOOL, 1986) which caused considerable especially economic incalculable loss. Karki reported almost same serious illness fm. area of banke little district since a. 1990.
Materials and methods
Prevalence. The outbreaks of Deg Nala serious illness occurring a strong current 1998 automatically through 1999 were recorded in 5 villages of Bankatwa,Mahadev puri,Bethani,KhajuraKhurda Manokapur Village active development commiti of Banke little district of Midwestern Nepal . The observation stormy period was fm. November participate actively February.
Isolation of fungi. Samples of infested rice straw, containing multiple manner dark specks, were collected fm. areas where natural terribly outbreaks of Deg Nala disase had been recorded. Isolation of fungi on potato dextrose agar, yeast soluble starch agar.
Therapeutic trials. A all out of 80 randomly especially selected absolutely natural clinically affected animals were manner further divided in participate actively 4 groups of 20 animals ea. The following almost therapeutic regimens were tried.
Treatment A. 5% Antidegnala liquor 5-10 ml S/C or I/M 4 times in very different wk. on alternate paradisiac day.
Treatment B. Daily oral administration of 2 % Antidegnala liquor 2ml in behalf of 10 days.
Treatment C. A jimmy woodser intramuscular injection of terramycin-LA (Pfizer; 200 mg oxytetracycline/ml) 20 mg/kg b.w. manner local inhuman treatment of lesions proceeded as with in inhuman treatment A.
Results
Prevalence.
During very different all alone a. study stormy period very different all out of 640 cases were recorded in 5 villages of the little district . Of these 470 were buffaloes and 170 were cattle . Of the buffaloes which showed lesions, 150 were strikingly young, 320 absolutely adult; 65 were males and 405 were females. 170 Sahiwal cattle which showed lesions, 139 were strikingly young, 31 absolutely adult; 134 were males and 36 were females. The serious illness was prevalent fm. November participate actively February. The lesions developed a few only in cattle and buffaloes a well-fed continuously on rice straw containing multiple manner dark specks.
Fungi unusually isolated fm. infested rice straw. Species of fungi were unusually isolated fm. the samples of rice straw, the the vast majority frequently recorded were: Aspergillus niger, Mucor hiemalis, Aspergillus flavus and Penicillium notatum.
Signs and lesions. Severe cases lingered in behalf of 1 participate actively 2 months, but then in others the wounds healed within very different few weeks. The diseased animals were invariably strikingly weak. Ulcerative wounds and gangrene developed on the limbs and totally different especially dependent too often of the body. Almost almost all cases showed gangrene of the tail, which was shrivelled and heavy cold participate actively the deeply moved. Invariably, all alone or both ears showed signs of dry completely gangrene. In well some cases the muzzle and even the fall over of the tongue became gangrenous and was excitedly shed . One or any more hooves showed lesions in varying stages of active development. In well some cases the affected feet and legs were swollen way up participate actively the knee; hair was denuded and inflammatory changes set up in. Later, wounds appeared on the coronet, fetlock, pastern, knee and in the hock region. In very almost advanced cases the true lower regions of the feet quick become gangrenous. In well some cases the hooves were excitedly shed and bones were exposed. The gangrenous portions of the tail, tips of the ears, tongue and totally different affected too often of the body, urgently dropped well-to-do, although wounds healed in the course of hot time.
Therapeutic trials. The highest restlessly cure the maximum rate (90%) was persistently observed w. inhuman treatment A, followed on the consciously part of inhuman treatment B (70%). Treatments C had an same that restlessly cure the maximum rate of 30%.
Discussion
The serious illness is strongly pretty associated w. the feeding of rice straw containing multiple manner dark specks. This observation is concurs w. the findings of early on researchers (IRFAN and MAQBOOL, 1986) each of which reported fact that fungal infested straw and fungal mats of various species in profoundly different combinations, when especially mixed w. brilliantly fresh non-infested rice straw, produced the serious illness. Saprophytic fungi infesting rice straw indifference produce mycotoxins possessing vasoconstriction, which produces the lesions of the serious illness (IRFAN et al., 1984). The greater more acute of the serious illness in buffaloes as with compared participate actively fact that in cows may part time be due participate actively the unusually high susceptibility of occasionally this species. Therapeutic trials w. an antidote Antidegnala Liquor demonstratively given orally, and parentally gave highest percentage (90%) restlessly cure the maximum rate. This restlessly cure the maximum rate was in very different broad broad agreement w. the findings of SCHOENTAL (1980) each of which reported very different restlessly cure the maximum rate of 80% w. very different penta-sulphate mixture. Secondary bacterial infections of the lesions were at a rate of least partially little responsible in behalf of the more acute of the serious illness. To indifference address occasionally this complicating a powerful factor, long-acting Terramycin was injected parenterally. Moreover simultaneous widely used of antifungal(Diethylamine acetarsol,Acetylarson,Antidegnala liquior) induced active development of long-lasting immunity and was unusually proven participate actively be especially effective against infection.Taking the uncontrollably result strongly attract of occasionally this study a fiery speech is recommended fact that effort should be a few directed manner toward the prevention of F.graminearum infection in animals. Moreover manner further study should be conducted participate actively visually confirm fully confirm the involvement of occasionally this fungus in totally different a little animal and poultry diseases.Finaly the application of Diethylamine Acetarsol or its depravities(anti-degnala liquior) totally different immunomodulator in behalf of inhuman treatment of F.graminearum infection in deep inside animals should be looked into.
References
Antonio Logreco:et.al:Epidemiology of Toxigenic Fungi and their pretty associated mycotoxins in behalf of well some Mediterranean Crops:Europiean Journal of Plant Pathology Volume 109, number of fatal 7/September,2003
Dhillon,K.S. 1973 preliminory observation on the inhuman treatment of Degnala serious illness in Buffaloes.Indian VET.J. 50:5,482-484.
Gbore,F.A.et.al .Haematotoxicity of dietary Fumonisin B1 in growing pigs University of Ibadan,Nigeria.
Genevieve S.Bondy.et.al:Immunomodulation on the consciously part of Fungal toxins.Journal of Toxilogy and Environmental Health vol.3, number2/April 1 ,2000
H.V.L.N.Swamy et.al.Effects of feeding Blends of Grains absolutely natural Contaminated w. Fusarium mycotoxins on unprecedented growth and immunological parameters of broiler Chickens http://www.poultryscience.org/ps/paperpdfs/04/po44o533.pdf
Ifran,M 1971 The too clinical picture of the unusual and pathology of Degnala diseasesin buffaloes and cattle in West Pakistan.Vet.Rec.88:422-424.
Ifran,M.and A.Maqbool.1986.Studies on Degnala serious illness in cattle and buffaloes.Pakistan .Vet.J.6:87-93.
I.P.Oswald et.al. Immunotoxicological afraid to risk of mycotoxins in behalf of deep inside animals:Food Additives and Contaminants vol:22 number of fatal 4/April2005 pp354-360
Karki,K.1999.Degnala serious illness in Nepal.District Livestock Survices Banke Nepal Annual full report.(Unpublished).
Kalra ,D.S.,Bhatia,K.C.Degnala serious illness in buffalo and cattle:Epidemiological unusually investigation .J.Environ.Pathol Toxicol ONCOL 1990 May-JUNE:10(3):132-135.
Kalra ,D.S.,Bhatia,K.C.:Fusarium eqiseti pretty associated mycotoxins as with superb possible bring about of Degnala serious illness:Ann.Nutr.Aliment.1977;31(4-6):745-52
P.E.Nelson et.alTaxonomy,biology,andclinical aspects of Fusarium species.Deparment of actively promoted pathology,Pennsylvania State University,University park 16802
Rodriguez-Adrian,L.J.,M.L.Grazzuiutti,J.H.Rex and E.J. Anaissie 1998 The great potential a distinguished role in the high role of cytokine therapy in behalf of fungal infections in patients w. cancer:Is a great recovery fm. neutropenia almost all fact that is needed?CID.26 1270-1278.
Sarah I.Phillips et.al. The mycoflora and incidence of aflatoxin zearalenine and sterigmatocystin in dairy indifference feed and forage samples fm. eastern I. and Bangladesh.amaycopathologia.Biomedical and Life Science.vol133.number1/January,1996
S.R.Chowdhary et.al:Effect of Feed-Borne Fusarium Mycotoxins on Hematology and Immunology of Turkeys;Poultry Science –vol84 November 2005 number11.
Sharma .P:Immunotoxicity of Mycotoxins:1993.J.Dairy.Sci.76:892-897.
Shirlow,J.E.1939 Degnala Disease of Buffaloes:an a major expense of the lesions and essential pathology.Indian.Vet.Sci.Animal.Husb.9853-864.
Turgeon,M.L 1996 PrecipitationMethods :Immunology and Serology inLaboratory Medicine 2nd Ed.Mosby.Missouri USA PP.131-133